| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072912 |
| Chromosome: | chromosome 3 |
| Location: | 4434282 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175400 | PGI,PGI1 | Phosphoglucose isomerase 1; (1 of 1) 5.3.1.9 - Glucose-6-phosphate isomerase / Phosphosaccharomutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGGCATCCGGCTCTGGTCGCCTCTACCGGTCCTCCCGAGGGCTGCC |
| Internal bar code: | GACAATACAACGTTGCCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3329 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 121 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACGGCTTCAACTACAGC |
| Suggested primer 2: | CACACACACACACAGCTTCG |