Insertion junction: CLIP2.072921_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072921
Chromosome:chromosome 14
Location:3944147
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre14.g632847 ELG44 Exostosin-like glycosyltransferase 44; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGCGGCTGCAGTGCTCCTTGCAGCGCGACTGTTTCCTTACGGGACTGG
Internal bar code:GGAGTCGGGGTGTGAGCTTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:88
LEAP-Seq percent confirming:43.4783
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCGATTGTGTGTGTGTT
Suggested primer 2:CAACTCGCAACTGCAACTCC