Insertion junction: CLIP2.072923_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.072923
Chromosome:chromosome 3
Location:8948966
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g800412 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATAACTGGGCACGCACAGGTTTGCATGTTGTCTTCCCTTGTGGCGTT
Internal bar code:TTGGTGCCTGTACTCCAGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:278
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCAGTCGGTCGCAACAAT
Suggested primer 2:GTCACACACACACACACACG