| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072927 |
| Chromosome: | chromosome 11 |
| Location: | 3705128 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478800 | (1 of 1) PTHR31027//PTHR31027:SF2 - FAMILY NOT NAMED // PROTEIN F18H3.4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGCAGCTATAGGGTGGAGTCGTGTTGATTGTTTTCGGTCTGCAGTC |
| Internal bar code: | CTAGCGATTACCGAAATTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1876 |
| LEAP-Seq percent confirming: | 75.5556 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGGCGAGAAGTTTGTC |
| Suggested primer 2: | CGTGCTCCCTGGTTGTTTTG |