| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072936 |
| Chromosome: | chromosome 9 |
| Location: | 4435239 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400553 | TOR1 | (1 of 1) K07203 - FKBP12-rapamycin complex-associated protein (FRAP); Target of rapamycin 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCCCTCCACTCTCCCTTGTTTGGGCTGGTATTTACACCACCACCACC |
| Internal bar code: | GGTAGCAGCGTTAGCATCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 5.7971 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGAGGGGAGAGGGAAGG |
| Suggested primer 2: | TTGTGACCTTTCATGGCCGA |