| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072944 |
| Chromosome: | chromosome 12 |
| Location: | 9150040 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543650 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGCATTTGCGCTTGCATCGGGCCACGATGGCGTTTTTATTCTGGCAT |
| Internal bar code: | CCGTCACGGTTTCGGAGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2048 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCAAAGCCCAGCGCATA |
| Suggested primer 2: | GCTGTCATGCAGTCCTCCAT |