| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072947 |
| Chromosome: | chromosome 12 |
| Location: | 5785491 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGACTACTGAAGGTTTTGGACATGTGAGAGTATATCACGGTTTATAAT |
| Internal bar code: | TTACTGGTGACCTTGTAAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGCGATTACTCTACGGA |
| Suggested primer 2: | GCTGTAGTATGGCGACTCCC |