Insertion junction: CLIP2.072953_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072953
Chromosome:chromosome 9
Location:3096624
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394065 FAP414 (1 of 2) K18635 - protein SPIRAL1 and related proteins (SPR1); Flagellar Associated Protein 414 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGACTCTACCAGCACAGCGTGAATACTCTCTCACCTTAACAGTTTATT
Internal bar code:TACTTGATCGGTGGTTGGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3302
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTACTTTGCCGACGACAA
Suggested primer 2:TCAGCCAAAATTGCAAGGGC