| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072955 |
| Chromosome: | chromosome 12 |
| Location: | 3867928 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515200 | DRC6,FAP169 | Nexin-dynein regulatory complex 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGGTACTCGGGTAGCTGTGCGAGTGGAATATGGCAGTTGTACAGAGG |
| Internal bar code: | CGTAATAGTCCCTTCTATACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1588 |
| LEAP-Seq percent confirming: | 22.7273 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACTTAGCACCGACCT |
| Suggested primer 2: | ACCAATGAGTAAGGGCTGCC |