| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072963 |
| Chromosome: | chromosome 3 |
| Location: | 1038538 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800291 | (1 of 9) 3.2.1.78 - Mannan endo-1,4-beta-mannosidase / Endo-1,4-mannanase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAATGCCAGCAGCAGCGTAAAGAGCGGCAGTAGCGGCACTAGTGGCA |
| Internal bar code: | GTAGATCATGATAATCACCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 2.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTCAGAGAACAGGTTCC |
| Suggested primer 2: | GGTGACTACCGAACACTGCA |