Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.072966 |
Chromosome: | chromosome 2 |
Location: | 8600378 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g800277 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTACAAAGAAGGGACAACCTTATGCTACCTTATGGTTGCGATATTACA |
Internal bar code: | GAATTTGGCTGCGACACGTTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2575 |
LEAP-Seq percent confirming: | 3.0303 |
LEAP-Seq n confirming: | 1 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACTGTTTCTCCCGTGCTGA |
Suggested primer 2: | CGCAAAGCACTGCTCGTATC |