| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072971 |
| Chromosome: | chromosome 17 |
| Location: | 4490208 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731150 | (1 of 2) PTHR22811//PTHR22811:SF31 - TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN // RE49489P | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCCCACTGCACCCAGCTACCGGCGGTCACGCAGGGCGTGGGCGCAC |
| Internal bar code: | TTTGACTCGAGGGTTTGATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1081 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAATTCACACGTGTGCC |
| Suggested primer 2: | CATCGCCTTGGCACATTAGC |