| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072972 |
| Chromosome: | chromosome 2 |
| Location: | 762321 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078300 | GPX1,PHGPx1 | Glutathione peroxidase 1, selenoprotein; (1 of 4) 1.11.1.9 - Glutathione peroxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGCGTGCGGGCCGCGGCGCCATGGAGCGTTGCTTCGGGAGGAGGCC |
| Internal bar code: | GTCTCAATTGCCTAGGAAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2914 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACAGTGGCACAAATCCG |
| Suggested primer 2: | CTTTGGCAAGTTCCTGGTGC |