Insertion junction: CLIP2.072975_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.072975
Chromosome:chromosome 12
Location:2610288
Confidence (%):40
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTGTCCAGGCGCCAATGAAAGGCTCTGCATCCCTACTGCTAATATGG
Internal bar code:CTGATTGAAGGGGGTGCTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:840
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAACAACCGCCCACGATTTG
Suggested primer 2:CAACACCACCTTTCACTGCG