| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.072988 |
| Chromosome: | chromosome 11 |
| Location: | 1546725 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468450 | CEN1,VFL2,DLE2 | 20 kD Calcium-Binding Protein Centrin (caltractin); (1 of 1) K16465 - centrin-1 (CETN1) | 3'UTR |
| Cre11.g468500 | MPA8 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR22953//PTHR22953:SF46 - ACID PHOSPHATASE RELATED // INACTIVE PURPLE ACID PHOSPHATASE 2-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACATTGATGCCCTGATCTTCTAGCTTTCGTCTTCAGGTACCAGGACG |
| Internal bar code: | TGGAATTTCAGTATTTATTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCACGATGTTGGGGTAG |
| Suggested primer 2: | CTGACTTGTCCACACGCTCT |