| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072994 |
| Chromosome: | chromosome 3 |
| Location: | 8923852 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204689 | PDE21 | (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | 3'UTR |
| Cre03.g204801 | (1 of 1) K10606 - E3 ubiquitin-protein ligase FANCL (FANCL, PHF9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATACACAAGCAAGCATGCATCCCATTGCGCACTGAACGCATGTGCTTA |
| Internal bar code: | TTTGAGAGAAGTCCCACCTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4226 |
| LEAP-Seq percent confirming: | 89.5238 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCCTGCCATACATGCTAG |
| Suggested primer 2: | TGAAAGGGGCAGTCTTCGTC |