| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073001 |
| Chromosome: | chromosome 10 |
| Location: | 5974363 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461350 | BUG23 | Basal-body proteins with upregulated genes 23 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGCGCATGCTGCTGCAGCGGGACCACCAGCTTTGGCTTTCAGCTGG |
| Internal bar code: | GATTTATACTATGGATCGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGATGGCAGGCATGGAA |
| Suggested primer 2: | TCCTTAACAGCCGCCAGAAG |