| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073004 |
| Chromosome: | chromosome 15 |
| Location: | 846785 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCACCACGCGCAGTGGTGTGTAAGACTGCCTGATCAGACCAACGACC |
| Internal bar code: | CGGGTACTGGGAGTTAATCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 7.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAACTGTGAAGCGGTAC |
| Suggested primer 2: | TGCTGTCTGCTTGGGGTATG |