Insertion junction: CLIP2.073009_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.073009
Chromosome:chromosome 2
Location:4880741
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g109683 (1 of 3) IPR002912//IPR011598 - ACT domain // Myc-type, basic helix-loop-helix (bHLH) domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACACAATGCGGTAGCACGAATCCCTGCAAGTGATGTGTGGACGTGTG
Internal bar code:CGCGGTTCGGTTGGTGAGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2147
LEAP-Seq percent confirming:73.1343
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:TAAACAAAGTGCAACCCGCG
Suggested primer 2:TGTCGAAAGGTGACTCCGTG