| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073009 |
| Chromosome: | chromosome 13 |
| Location: | 645408 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566000 | FTL1 | Putative monofunctional formate-tetrahydrofolate ligase; (1 of 1) 6.3.4.3 - Formate--tetrahydrofolate ligase / Tetrahydrofolic formylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGATCCGCACCCTGCCCTCTGCCCACACGCCAGCCACTCTGACCTGT |
| Internal bar code: | TTTGCAGCGCAGCGTGCAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCTAACACACCCAACCT |
| Suggested primer 2: | ACAGGTAGCTAGGCTCGTGA |