| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073015 |
| Chromosome: | chromosome 6 |
| Location: | 3048204 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274750 | HTB6,HTB4,HTB26 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTCTTCTACTCTCAACAAAACTCGGTGTTTCTCAACACCACCTTGAC |
| Internal bar code: | TGGACCGGTCTAGGTTCACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTTTTGGTGTCAGGCAG |
| Suggested primer 2: | TCTGCCGCATCAACAACTCT |