| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073020 |
| Chromosome: | chromosome 12 |
| Location: | 2671224 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505300 | (1 of 1) PF08317 - Spc7 kinetochore protein (Spc7) | 3'UTR | |
| Cre12.g505350 | FAP10,ACA2 | Flagellar Associated Protein 10; (1 of 2) PTHR24093:SF254 - PLASMA MEMBRANE CALCIUM-TRANSPORTING ATPASE 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCTCGGGCTACTTGTGTGTACAAAGGACTAGCGATGTTGTCTCATGT |
| Internal bar code: | GGCATTGCCCCTGACTGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3552 |
| LEAP-Seq percent confirming: | 47.8873 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCCGTGTGTTCAACAAC |
| Suggested primer 2: | TGTTGTGTCCGTAAGCCTCC |