| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073024 |
| Chromosome: | chromosome 8 |
| Location: | 2856171 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374050 | DIV28,POLD2 | (1 of 1) K02328 - DNA polymerase delta subunit 2 (POLD2); DNA polymerase delta, small subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCGCCATGGTGCCGGGTGTCGCCACACTCAGCACCATACGCGCGCC |
| Internal bar code: | TGATTTTTTTTTTTTGACCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTGGGATGGTCGTGTT |
| Suggested primer 2: | ATGTGCCTGAGCCCAATGAA |