| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073027 |
| Chromosome: | chromosome 4 |
| Location: | 2323004 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220825 | (1 of 3) PF10601 - LITAF-like zinc ribbon domain (zf-LITAF-like) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGATTAAAAGTTAACTTGCTTCGCAGCACGAATGACCCCTGAAGTCCC |
| Internal bar code: | TGCAGTATAGTCGTAGCAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1682 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCCCTGTCGGTAAGCAA |
| Suggested primer 2: | CAACCCCGACACGACTAACA |