| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073037 |
| Chromosome: | chromosome 13 |
| Location: | 35944 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g561850 | FRK2 | Fructokinase; (1 of 1) 2.7.1.4 - Fructokinase / D-fructokinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATCCTGCCTGTAGTCGGCAGTACCGCCATGGTTGGGCCGTTGTTGGC |
| Internal bar code: | TATAAAAATGTCATATCATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4262 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACATCCCTAAGCCGTAC |
| Suggested primer 2: | AGGGGCATGCACATGATTGA |