| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073040 |
| Chromosome: | chromosome 3 |
| Location: | 9251690 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800424 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGCTCGATCCTCTGACCTCTCGATTACACCATGTCCGATTCTGAGT |
| Internal bar code: | TAAATCGCAGATAGGCATATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 18.75 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTACTCCTTGCTGTGGG |
| Suggested primer 2: | AGCCCCTAGTGCTGTACGTA |