Insertion junction: CLIP2.073056_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.073056
Chromosome:chromosome 11
Location:3249798
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre11.g476250 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCGCTTCCGCTCTCGCTGCCTCCCGCCACCCACCAGTTGCCGTTGCCG
Internal bar code:GGATAATGTCCGGGTTAGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:861
LEAP-Seq percent confirming:84.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGTGTACTTCCTGGTGG
Suggested primer 2:TATGCCCTGTAGCGTGTGTG