Insertion junction: CLIP2.073078_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.073078
Chromosome:chromosome 9
Location:4108028
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399252 3'UTR
Cre09.g399289 (1 of 3) PF00651//PF03110 - BTB/POZ domain (BTB) // SBP domain (SBP) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCATGATGACCGCACCCGCATCCTTCTCAGGCCAAGCGGCACTCTCA
Internal bar code:GGAGCAGTGTCTTGAGAGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2269
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGACGAGGAGGACGAAC
Suggested primer 2:TTACGGGTTCAGTCGGTGTG