Insertion junction: CLIP2.073080_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.073080
Chromosome:chromosome 6
Location:284621
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g250800 CRB1 (1 of 1) K13210 - far upstream element-binding protein (FUBP); C1, subunit of the circadian RNA-binding protein CHLAMY1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGAAATCTGCACCTCCTCCCTGCCATCCGCCTACCCGTCCGCCTGTTC
Internal bar code:TCTTCTTGGGGTAGGTAAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1027
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGACCACGGTTGTGTTGTTC
Suggested primer 2:ATTGCGCACAAAACCCTTCC