| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073087 |
| Chromosome: | chromosome 13 |
| Location: | 5207531 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607600 | FSN1,RIBFL1 | Similar to riboflavin biosynthesis protein RibF; (1 of 1) PTHR22749//PTHR22749:SF0 - RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE // RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBF | 3'UTR |
| Cre13.g607650 | POC15 | Proteome of centriole protein 15; (1 of 1) PTHR10891//PTHR10891:SF5 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAGACGCATTATTTTAACTTGCAACGGCGGCAAAGACCTCGTCGGCAG |
| Internal bar code: | GCATACTCTGTGAAATAGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1722 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGACGCTGAGCCTCTA |
| Suggested primer 2: | AGACTTCGATGCATGCAGCT |