| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073093 |
| Chromosome: | chromosome 16 |
| Location: | 2698684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661750 | (1 of 2) PF08332 - Calcium/calmodulin dependent protein kinase II association domain (CaMKII_AD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGGGACTGAATGACACGGTGGTCCGGATGGCAGGGTATTCCTGCGG |
| Internal bar code: | GGTAGGGATGGCCTCTCCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCACAGTCGCGCTAAGC |
| Suggested primer 2: | CCGCCGAGATTGAGGACTAC |