Insertion junction: CLIP2.073097_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.073097
Chromosome:chromosome 3
Location:7243539
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre03.g207700 FPPS1,FPPS,PPS1,FPS1 (1 of 1) 2.5.1.1//2.5.1.10 - Dimethylallyltranstransferase / Prenyltransferase // (2E,6E)-farnesyl diphosphate synthase / Geranyltranstransferase; Farnesyl pyrophosphate synthase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATAAATTACATATTGCATGAATTTCCTGTGCGGTTCACTTATTCGCAG
Internal bar code:GTCACAGGGCGGTTGGGCACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1568
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTCGTATACCGCCATTCC
Suggested primer 2:TGCTGTGGTGGCATAGTGAG