| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073099 |
| Chromosome: | chromosome 2 |
| Location: | 1740651 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800182 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCGATCATTTACTTTACGGCCTTCAGGCGCGTATCGCACATACGGCC |
| Internal bar code: | TCTACTGCGTCGCATCGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1150 |
| LEAP-Seq percent confirming: | 35.2941 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTCTCGAGCAGGCTAAG |
| Suggested primer 2: | GCGAAACATCGTCAGCGTAC |