| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073117 |
| Chromosome: | chromosome 2 |
| Location: | 2282824 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800195 | (1 of 2) K01148 - poly(A)-specific ribonuclease (PARN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTCACTCCGGAGTCCCTTGCCGGGATCGACCTATCTATCGGCGCATC |
| Internal bar code: | TAGTTGGGAGGTGTTATTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 5.71429 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGGCGAAATGTAATGGGT |
| Suggested primer 2: | GATCCGGACAAGCATTTGGC |