| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073121 |
| Chromosome: | chromosome 10 |
| Location: | 4471860 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450879 | (1 of 1) K14859 - ribosome biogenesis protein SSF1/2 (SSF1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCCCGCTTCTCACGGCTACCCCTTCCCCTTCCCCTTCACACACACA |
| Internal bar code: | TTGTTGAGCTCTGGCCGGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCGAGTTCGTGACCAAG |
| Suggested primer 2: | ACAGACCCTCCTCCACCTG |