Insertion junction: CLIP2.073129_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073129
Chromosome:chromosome 16
Location:3386726
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACGCCCCAACCCGCTGCCCCCACCAGCCTGCTGTCCCCCTGCCGGCC
Internal bar code:AGCCACTTTGGAGTGGATCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:63
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTACATGGCGGACCTCCATC
Suggested primer 2:GCGTCTAGGAGCACGTAGTC