| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073131 |
| Chromosome: | chromosome 6 |
| Location: | 3328527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277700 | (1 of 1) 2.7.1.108 - Dolichol kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCACACATAAATAATGAACCCGTATACAAATAATGGACGTTTTGTTAA |
| Internal bar code: | TGATGTGTGTCATGAGAGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1171 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGGTGTGAATGCAGGCC |
| Suggested primer 2: | TCTCCTCGAAGCGCTTCTTC |