| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073132 |
| Chromosome: | chromosome 12 |
| Location: | 3783554 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514450 | SPL20 | Pre-mRNA splicing factor; (1 of 1) K12829 - splicing factor 3B subunit 2 (SF3B2, SAP145, CUS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAATTTGATGGATGGGTAAGGTGGAGCACAGTGAGAAAAGGGTTTCT |
| Internal bar code: | TCCGGGCTGTGGCTATAGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGAAGTCCGCAGTGGGAA |
| Suggested primer 2: | CAGCTGGCAATGCAGCATAG |