| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073138 |
| Chromosome: | chromosome 16 |
| Location: | 7641997 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685053 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCTTTGCGACGTCAGCGCAGGCCTCCAACCCGCCTCCTCCCCCTTCT |
| Internal bar code: | GCTCTTCGCTGGTTCGATAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 10.2564 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGGCTGCAGATGATGAT |
| Suggested primer 2: | AGGACTACGTGGGTATGGCT |