Insertion junction: CLIP2.073139_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.073139
Chromosome:chromosome 10
Location:530854
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g421200 (1 of 3) PF01985 - CRS1 / YhbY (CRM) domain (CRS1_YhbY) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCTCGACGTCGGCGACGGCTACAGAAAATGTTGCCGATGATTTCGATG
Internal bar code:AGCGTCCGCTGGCGCACTTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:878
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATAGGGGTAAGGGGGTT
Suggested primer 2:GCAAGGGCAAGTAGGATGGT