Insertion junction: CLIP2.073149_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.073149
Chromosome:chromosome 6
Location:6256270
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292249 PHC50 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGAGCGGAGTGACCACAACTACGCAGCCTGTTTCGCCTACTGTTTTGC
Internal bar code:TAGAATAGGGTCTGGTTTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1874
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCTCCTGGGTCTCAGTA
Suggested primer 2:TTTCGTGCGGTCTCGTGTAA