| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073160 |
| Chromosome: | chromosome 8 |
| Location: | 3395215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377950 | (1 of 3) PF02622 - Uncharacterized ACR, COG1678 (DUF179) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACTCAATTCCAACAAACGGACATACGGGAAAACCACGCCACACTAGC |
| Internal bar code: | ATTTTGGACGTACTGAACGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2001 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACACGTGGCTGTAGGT |
| Suggested primer 2: | AGCCACCTCCATCAGATCCT |