| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073163 |
| Chromosome: | chromosome 6 |
| Location: | 7760151 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303000 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAATGCCAAACCCGGCACACGACGTGGCGCACAAACATGAATCACCCGG |
| Internal bar code: | ATGGATACATCGTAGAAGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 2.63158 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTAACCCCTACCACCCG |
| Suggested primer 2: | ACACACCTATCGCATAGCCG |