| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.073167 |
| Chromosome: | chromosome 12 |
| Location: | 6989032 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560902 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACAAGAAGCGAAGACGAATCGTCGGTCGAATAATACAACCTTGGCC |
| Internal bar code: | GATCGTGTGTAATAGACTTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1943 |
| LEAP-Seq percent confirming: | 1.75439 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCACAGCCACAGCCATA |
| Suggested primer 2: | AGTGATTCGCGAGGTTCTGG |