| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073171 |
| Chromosome: | chromosome 10 |
| Location: | 5193874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGAATGTAGCGATTACAGCGAAGGTCAGTCAGTCAACAAAGTAAGGC |
| Internal bar code: | AGGAAAGTCCTAGAGGTCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1140 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGGAGAGCAGATGCCTG |
| Suggested primer 2: | TGTTGTTATGCGGCTTGTGC |