| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073172 |
| Chromosome: | plastome |
| Location: | 135939 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802320 | chlN,2716986,ChreCp056 | light-independent protochlorophyllide reductase subunit N; (1 of 1) K04038 - light-independent protochlorophyllide reductase subunit N (chlN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCCTTAGCATCCTTAAAAAAAGATGTTCCTTTTTTAGTAGGTAATAC |
| Internal bar code: | GACCGCGCCCGGCTGATTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 1.72414 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCGGGGCCTATTGGAAA |
| Suggested primer 2: | GTGGCAGCTCGTCTTCAGAA |