| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073174 |
| Chromosome: | chromosome 8 |
| Location: | 1867244 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367300 | ESS101 | (1 of 2) PF17035 - Bromodomain extra-terminal - transcription regulation (BET) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGCAATTATGTGCAGGTATGCATCGGGCAACAGGTACCAAGCAAAG |
| Internal bar code: | AAGCCCGCAATTCACAGTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1504 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTGGCATGTTGCGTTGT |
| Suggested primer 2: | AGCGTTGAGAAGCGTGGTAA |