| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073174 |
| Chromosome: | chromosome 8 |
| Location: | 1867249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367300 | ESS101 | (1 of 2) PF17035 - Bromodomain extra-terminal - transcription regulation (BET) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAACGCCCGGAATGTGGCGTGCGAGGTTGTAAAAACGATGGCGCACTGC |
| Internal bar code: | CGGGTGGCAGAGTCAGGTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTTGAGAAGCGTGGTAA |
| Suggested primer 2: | GGATTGGCATGTTGCGTTGT |