Insertion junction: CLIP2.073181_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.073181
Chromosome:chromosome 6
Location:6443893
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g293700 MRPL7,L12 Ribosomal protein L7/L12 family protein; (1 of 1) K02935 - large subunit ribosomal protein L7/L12 (RP-L7, MRPL12, rplL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCTGTATTAGATTGGCTCAACTGGCGTCCACAGCAGTCACCAGTGCC
Internal bar code:TGTACTGTACATAGGTACTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:1.72414
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGGTGAGAACAGTGTGA
Suggested primer 2:CCACGCTGGAACCTGTTTTG