| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073194 |
| Chromosome: | chromosome 17 |
| Location: | 733323 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700950 | FDX5 | (1 of 2) PTHR19370//PTHR19370:SF110 - NADH-CYTOCHROME B5 REDUCTASE // SUBFAMILY NOT NAMED; Ferredoxin 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTCCACCTGGCCCTCCTGCACACGGCCCGCGCACGTGCCGCAGGTGC |
| Internal bar code: | AGCACTTGTATATAGGTGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCGCTTTGGTCGCTGTG |
| Suggested primer 2: | CTTCAAGCCGCGTGTCAAAA |